Title : 1 Effect of Honey on Streptococcus mutans Growth and Biofilm Formation 2 3 Running Title : 4 Effect of Honey on Streptococcus
نویسنده
چکیده
34 Because of the tradition of using honey as an antimicrobial medicament, we investigated 35 the effect of natural honey (NH) on Streptococcus mutans growth, viability and biofilm 36 formation compared to an artificial honey (AH). AH contained the sugars at the concentrations 37 reported for NH. NH and AH concentrations were obtained by serial dilution with tryptic soy 38 broth (TSB). Several concentrations of NH and AH were tested for inhibition of bacterial 39 growth, viability and biofilm formation after inoculation with S. mutans UA159 in 96-well 40 microtiter plates to obtain absorbance and CFU values. Overall, NH supported significantly less 41 (p < 0.05) bacterial growth compared to the AH at 25 and 12.5% concentrations. At 50 and 25% 42 concentrations, both honey groups provided significantly less bacterial growth and biofilm 43 formation compared to the TSB control. For bacterial viability, all honey concentrations were not 44 significantly different from the TSB control except for 50% NH. NH was able to decrease the 45 maximum velocity of S. mutans growth compared to AH. In summary, NH demonstrated more 46 inhibition of bacterial growth, viability and biofilm compared to AH. This study highlights the 47 potential antibacterial properties of NH, and could suggest that the antimicrobial mechanism of 48 NH is not solely due to its high sugar content. 49 50 on Jne 9, 2017 by gest ht://aem .sm .rg/ D ow nladed fom
منابع مشابه
Effect of Sodium Fluoride , Ampicillin and Chlorhexidine on 1 Streptococcus mutans Biofilm Detachment 2 3 Running title : Streptococcus mutans biofilm detachment 4
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